cDNA & CLONING KITS

cDNA & CLONING KITS




Reverse Transcriptase is used to synthesize complementary DNA (cDNA) using RNA as a template. Enzynomics provides three types of reverse transcriptase, M-MLV, TOPscript ™, and SuperiorScript.

TOPscript ™ Reverse Transcriptase is a product that can synthesize cDNA at a high temperature of 60 ℃ by engineering M-MLV reverse transcriptase. This is very useful when the RNA template has a long, complex secondary structure.​

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cDNA


M-MLV Reverse Transcriptase

Allianz Bio, in collaboration with Enzynomics, offers the M-MLV Reverse Transcriptase and M-MLV cDNA Synthesis Kit, both featuring a recombinant reverse transcriptase derived from Moloney murine leukemia virus (M-MLV).

One-Step RT-PCR Kit

Allianz Bio offers the TOPscript™ RT-PCR Kit series, including RT-PCR Kit, DryMIX, DyeMIX, and DryDyeMIX, designed for fast, convenient, and high-yield one-step RT-PCR.

Improved Reverse Transcriptase

TOPscript™ and SuperiorScript™ offer high-efficiency reverse transcriptases and cDNA synthesis kits with enhanced thermostability and yield. DryMIX formats and ready-to-use master mixes enable convenient, reliable RT workflows.



Cloning Kits


TOPcloner™ The TOPcloner™ PCR Cloning Kit is designed to clone the insert DNA fragment generated by PCR directly into the pTOP Vector without ligase treatment.

It can minimize self-ligation and clone quickly in 5 minutes with high efficiency EZ-Fusion™ The EZ-Fusion™ HT Cloning Kit is a quick and easy way to replicate PCR-amplified DNA fragments to all cloning vectors.

EZ-Fusion™ recognizes the 18-nt complement sequence of the PCR product and the linearized vector DNA ends and combines them efficiently and precisely.

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List of Products -


TOPcloner™ TA kit TOPcloner™ TA core kit TOPcloner™ Blunt kit TOPcloner™ Blunt core kit
TOPcloner™ TA-Blunt kit TOPcloner™ TA-Blunt core kit TOPcloner™ XL TA Kit TOPspeed™ DNA Ligation kit
EZ-Fusion™ HT Cloning Kit EZ-Fusion™ HT Cloning core Kit EZ-Fusion™ HT Dry Cloning Kit EZ-Fusion™ HT Dry Cloning Core Kit
NEW EZ™ Multiplex Assembly Kit NEW EZ™ Multiplex Assembly Core Kit

Mutagenesis Kit

The EZchange™ mutagenesis kit series provides a versatile and efficient solution for introducing genetic mutations into DNA sequences.

These kits support both site-directed and random mutagenesis, allowing researchers to introduce single or multiple mutations, or generate mutation libraries for protein engineering and functional studies.

Designed for high accuracy and user convenience, they are ideal for applications such as gene function analysis, protein optimization, and directed evolution.

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EZchange™ Site-directed Mutagenesis kit EZchange™ Multi Site-directed Mutagenesis kit
EZchange™ Multi Site-directed Mutagenesis core kit EZ™ Random Mutagenesis Kit